Day 3 vs Day 5 Blastocyst Transfer

There are very different schools of thought as to whether to perform a Day 3 or Day 5 Blastocyst stage embryo transfer. Not all Day 2 or 3 embryos can develop to a blastocyst with a loss of about 45 – 60% of your embryos as they are cultured the extra 2 or 3 days. Therefore, those embryos that do make it to a blastocyst for transfer on Day 5 are considered the best or hardiest and it is a natural selection process. The problem that arises is that there may be nothing to transfer back. This is why we have the criteria we have in deciding which day to do the embryo transfers.

On Day 2, after egg retrieval, we can more accurately make decisions on whether to schedule a transfer on Day 3 or Day 5 based on the number of “good” quality eggs, 4-cell embryos that are developing when the embryos are evaluated. For patients under 37 years old we need at least 5 good quality 4-cell embryos. Some fertility clinics require more than 5, but 5 seems to work for us. We re-evaluate on Day 3, at this time we need 3 good quality 8-cell embryos to continue going to Day 5. If the following criteria are not met we will schedule the transfer for Day 4.

Now that the day-of-transfer has been discussed, the number of embryos to transfer needs to be approached. At The Center for Reproductive Medicine, we follow the ASRM guidelines of 2 embryos in patients under 37 years old; 3 embryos in patients 38-39 years old; and, 4 embryos in patients 40 and older on a Day 3 transfer. For a Day 5 transfer this number can be reduced by 1 embryo and should be reduced by 1 embryo in fertility patients that are under 35 in order to reduce the potential for multiple pregnancy risks. If the embryo quality is poor, or if there have been previous failed IVF cycles, the number transferred can be adjusted after thorough discussion with the reproductive endocrinologists.


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Dr. Koulianos leads The Center’s consistent delivery of the best science, medicine and care through its focus on human compassion, determination to help patients succeed.
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A skilled physician, Dr. Inge received his Medical Doctor degree from the University of Alabama School of Medicine in Birmingham, AL. He completed residency in Obstetrics and Gynecology at the Walter Reed Army Medical Center in Washington, D.C.